Hotspot

rps8

LSC · 78231-78635 · 405 bp

Back to Baptisia

Pi 0.00247

MarkerSeek score 42.70

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 1
pi: 0.00247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TCATTTGTGCATTCGGTCCT	CAATCGCCAGGGAATTGAGA	45.0	50.0	57.8	58.0	1341 bp	1.0	78.2
rps8_p2	TCATTTGTGCATTCGGTCCT	AATCGCCAGGGAATTGAGAC	45.0	50.0	57.8	58.0	1340 bp	1.0	78.2
rps8_p3	TCATTTGTGCATTCGGTCCT	GAACTCGAATTTTTGGCGCA	45.0	45.0	57.8	58.0	1359 bp	1.0	78.2
rps8_p4	TCATTTGTGCATTCGGTCCT	AATGATAATGCTGCGGTCGT	45.0	45.0	57.8	58.0	1405 bp	1.0	78.2
rps8_p5	TCATTTGTGCATTCGGTCCT	ATAATGCTGCGGTCGTCATT	45.0	45.0	57.8	58.0	1401 bp	1.0	78.2