Hotspot

rpl16

LSC · 79371-80891 · 1521 bp

Back to Baptisia

Pi 0.00131
MarkerSeek score 59.50
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1521
variable_sites
2
pi
0.00131
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TGCATCCTAAAGACCTCTTTCC AGGTTCCATATTTCGATAAAACA 45.5 30.4 57.8 54.1 1651-1656 bp 1.0 40.1
rpl16_p2 TGCATCCTAAAGACCTCTTTCC TCTTTAAGGTTCCATATTTCGA 45.5 31.8 57.8 52.9 1657-1662 bp 1.0 40.1
rpl16_p3 TGCATCCTAAAGACCTCTTTCC AAGGTTCCATATTTCGATAAAACA 45.5 29.2 57.8 54.8 1652-1657 bp 1.0 40.1
rpl16_p4 TGCATCCTAAAGACCTCTTTCC AGGTTCCATATTTCGATAAAACAA 45.5 29.2 57.8 54.8 1651-1656 bp 1.0 40.1
rpl16_p5 GCATCCTAAAGACCTCTTTCCT AGGTTCCATATTTCGATAAAACA 45.5 30.4 57.5 54.1 1650-1655 bp 1.0 40.1