Hotspot

ndhF

SSC · 108278-110581 · 2304 bp

Back to Baptisia

Pi 0.000434

MarkerSeek score 57.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2304
variable_sites: 1
pi: 0.000434
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACTATTTGGGCAGAACACCG	ACAAAAGAGATAAAACCGGCG	50.0	42.9	58.2	57.2	2423 bp	1.0	64.0
ndhF_p2	CTATTTGGGCAGAACACCGT	ACAAAAGAGATAAAACCGGCG	50.0	42.9	58.2	57.2	2422 bp	1.0	64.0
ndhF_p3	TATTTGGGCAGAACACCGTC	ACAAAAGAGATAAAACCGGCG	50.0	42.9	58.2	57.2	2421 bp	1.0	64.0
ndhF_p4	CTATTTGGGCAGAACACCGT	AACCGGCGAATAAGAAACAA	50.0	40.0	58.2	56.0	2409 bp	1.0	62.3
ndhF_p5	CTATTTGGGCAGAACACCGT	ACCGGCGAATAAGAAACAAA	50.0	40.0	58.2	56.0	2408 bp	1.0	62.3