Hotspot

ndhD

SSC · 112868-114367 · 1500 bp

Back to Baptisia

Pi 0.00133

MarkerSeek score 55.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1500
variable_sites: 2
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAGGGTTCTCACAAACACCA	AAAACCTGTTCTCAAAATAAAGA	45.0	26.1	57.2	52.7	1679 bp	1.0	40.1
ndhD_p2	AAGGGTTCTCACAAACACCA	TCTTTATTTTGAGAACAGGTTTT	45.0	26.1	57.2	52.7	1650 bp	1.0	40.1
ndhD_p3	AGGGTTCTCACAAACACCAA	AAAACCTGTTCTCAAAATAAAGA	45.0	26.1	57.2	52.7	1678 bp	1.0	40.1
ndhD_p4	AGGGTTCTCACAAACACCAA	TCTTTATTTTGAGAACAGGTTTT	45.0	26.1	57.2	52.7	1649 bp	1.0	40.1
ndhD_p5	AGGGTTCTCACAAACACCAA	TCTTTATTTTGAGAACAGGTTTTT	45.0	25.0	57.2	53.6	1649 bp	1.0	40.1