Hotspot

ndhA

SSC · 116888-119140 · 2253 bp

Back to Baptisia

Pi 0.00178

MarkerSeek score 48.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2253
variable_sites: 4
pi: 0.00178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTCTTTCTATTGTTTGAGACA	TGGGAGAAGTTGATCGTTGA	29.2	45.0	54.9	56.5	2324 bp	1.0	40.1
ndhA_p2	TGTTTCTTTCTATTGTTTGAGACA	TGGGAGAAGTTGATCGTTGAA	29.2	42.9	54.9	57.2	2324 bp	1.0	40.1
ndhA_p3	TTGTTTCTTTCTATTGTTTGAGACA	TGGGAGAAGTTGATCGTTGA	28.0	45.0	55.6	56.5	2325 bp	1.0	40.1
ndhA_p4	TGTTTCTTTCTATTGTTTGAGACA	ATGGGAGAAGTTGATCGTTGA	29.2	42.9	54.9	57.0	2325 bp	1.0	40.1
ndhA_p5	TTGTTTCTTTCTATTGTTTGAGACA	TGGGAGAAGTTGATCGTTGAA	28.0	42.9	55.6	57.2	2325 bp	1.0	40.1