Hotspot

clpP

LSC · 67969-69977 · 2009 bp

Back to Baptisia

Pi 0

MarkerSeek score 57.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2009
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	CCGGCGGTAAGAAGATCAAA	CGTTTCCACATCAAAGTGCG	50.0	50.0	58.0	58.3	2125-2126 bp	1.0	77.3
clpP_p2	GTATTTGCGCGGAATCCTTG	CGTTTCCACATCAAAGTGCG	50.0	50.0	57.9	58.3	2089-2090 bp	1.0	76.6
clpP_p3	TGTATTTGCGCGGAATCCTT	CGTTTCCACATCAAAGTGCG	45.0	50.0	58.3	58.3	2090-2091 bp	1.0	75.4
clpP_p4	CCCTTAAATCCGGCGGTAAG	CGTTTCCACATCAAAGTGCG	55.0	50.0	58.4	58.3	2134-2135 bp	1.0	74.2
clpP_p5	CCGGCGGTAAGAAGATCAAA	ACGTTTCCACATCAAAGTGC	50.0	45.0	58.0	57.2	2126-2127 bp	1.0	73.7