Hotspot

atpF

LSC · 27313-28666 · 1354 bp

Back to Baptisia

Pi 0

MarkerSeek score 58.80

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1354
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TGCGAATCCTTTTGTTTAATCCT	TGCACGAATGGTTACCATGA	34.8	45.0	57.0	57.5	1548 bp	1.0	44.4
atpF_p2	TGCGAATCCTTTTGTTTAATCCT	ATCTGCACGAATGGTTACCA	34.8	45.0	57.0	57.2	1551 bp	1.0	42.0
atpF_p3	TGCGAATCCTTTTGTTTAATCCT	CTGCACGAATGGTTACCATG	34.8	50.0	57.0	57.2	1549 bp	1.0	41.6
atpF_p4	TTGCGAATCCTTTTGTTTAATCCT	TGCACGAATGGTTACCATGA	33.3	45.0	57.7	57.5	1549 bp	1.0	41.5
atpF_p5	TGCGAATCCTTTTGTTTAATCC	TGCACGAATGGTTACCATGA	36.4	45.0	55.6	57.5	1548 bp	1.0	41.1