Hotspot

trnG-UCC-trnS-GCU

LSC · 31580-32335 · 756 bp

Back to Baptisia

Pi 0
MarkerSeek score 58.90
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
756
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 GGGGAATCAAACGGAGGATC CAATCCGACGCTTTAGTCCA 55.0 50.0 58.0 58.0 870-871 bp 1.0 79.8
trnG-UCC-trnS-GCU_p2 GGGGAATCAAACGGAGGATC AATCCGACGCTTTAGTCCAC 55.0 50.0 58.0 58.0 869-870 bp 1.0 79.8
trnG-UCC-trnS-GCU_p3 TTGATCGGGGAATCAAACGG CAATCCGACGCTTTAGTCCA 50.0 50.0 58.3 58.0 876-877 bp 1.0 78.0
trnG-UCC-trnS-GCU_p4 TTGATCGGGGAATCAAACGG AATCCGACGCTTTAGTCCAC 50.0 50.0 58.3 58.0 875-876 bp 1.0 78.0
trnG-UCC-trnS-GCU_p5 GGGGAATCAAACGGAGGATC GTGCGGAAAGAGAGGGATTC 55.0 55.0 58.0 58.3 925-926 bp 1.0 77.1