Hotspot

rbcL-atpB

LSC · 6177-6945 · 769 bp

Back to Baptisia

Pi 0

MarkerSeek score 59.10

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	AACACCAGCTTTGAACCCAA	CCTGGGGGAAAAGCTACATC	45.0	55.0	58.1	57.9	930-933 bp	1.0	78.7
rbcL-atpB_p2	AACACCAGCTTTGAACCCAA	CTGGGGGAAAAGCTACATCC	45.0	55.0	58.1	57.9	929-932 bp	1.0	78.7
rbcL-atpB_p3	AACACCAGCTTTGAACCCAA	GGGGGAAAAGCTACATCCAG	45.0	55.0	58.1	57.9	927-930 bp	1.0	78.7
rbcL-atpB_p4	AACACCAGCTTTGAACCCAA	AAGCTACATCCAGTACCGGA	45.0	50.0	58.1	57.8	920-923 bp	1.0	77.9
rbcL-atpB_p5	AACACCAGCTTTGAACCCAA	CTACATCCAGTACCGGACCA	45.0	55.0	58.1	58.2	917-920 bp	1.0	77.3