Hotspot

ycf1

SSC · 114592-119862 · 5271 bp

Back to Baphia

Pi 0.383
MarkerSeek score 74.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5271
variable_sites
1689
pi
0.383
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGGGCCCTCTTATCTCTTCC CACGACAGGGTCCAGTTAAT 55.0 50.0 57.9 57.2 1208 bp 0.5 53.0
ycf1_p2 GGGCCCTCTTATCTCTTCCT CACGACAGGGTCCAGTTAAT 55.0 50.0 57.9 57.2 1207 bp 0.5 53.0
ycf1_p3 GTCGTTGTGGTCGGACTTTA CACGACAGGGTCCAGTTAAT 50.0 50.0 57.9 57.2 1255 bp 0.5 52.8
ycf1_p4 ATGAATTCGGTCGTTGTGGT CACGACAGGGTCCAGTTAAT 45.0 50.0 57.8 57.2 1264 bp 0.5 52.5
ycf1_p5 CTCTTCCTTCTTCGAGCTCG CACGACAGGGTCCAGTTAAT 55.0 50.0 57.8 57.2 1195 bp 0.5 52.4