Hotspot

ndhH

SSC · 120613-121794 · 1182 bp

Pi 0.483

MarkerSeek score 80.40

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 477
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GAAAACCCGGGATCTGTTGA	TCAGGTCCAATACGCTGTTG	50.0	50.0	57.8	57.9	1709 bp	0.5	57.7
ndhH_p2	AGAAAACCCGGGATCTGTTG	TCAGGTCCAATACGCTGTTG	50.0	50.0	57.8	57.9	1710 bp	0.5	57.7
ndhH_p3	GAAAACCCGGGATCTGTTGA	GCTCGTAGACCACCCAAAAA	50.0	50.0	57.8	58.1	2006 bp	0.5	57.5
ndhH_p4	AGAAAACCCGGGATCTGTTG	GCTCGTAGACCACCCAAAAA	50.0	50.0	57.8	58.1	2007 bp	0.5	57.5
ndhH_p5	GAAAACCCGGGATCTGTTGA	CAGGTCCAATACGCTGTTGT	50.0	50.0	57.8	58.2	1708 bp	0.5	56.9