Hotspot

ndhD

SSC · 126780-128279 · 1500 bp

Back to Baphia

Pi 0.481
MarkerSeek score 79.40
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1500
variable_sites
578
pi
0.481
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.80
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 ATTTTCCATTTTGAGCGCGG TCAGGAGCAGTATGGGCTAA 45.0 50.0 58.0 57.8 2088 bp 0.5 58.6
ndhD_p2 ATTTTCCATTTTGAGCGCGG TTCAGGAGCAGTATGGGCTA 45.0 50.0 58.0 57.8 2089 bp 0.5 58.6
ndhD_p3 AAACAACTCGAAGCATGGGT TCAGGAGCAGTATGGGCTAA 45.0 50.0 58.0 57.8 2182 bp 0.5 58.5
ndhD_p4 AAACAACTCGAAGCATGGGT TTCAGGAGCAGTATGGGCTA 45.0 50.0 58.0 57.8 2183 bp 0.5 58.5
ndhD_p5 GGCATGAAACAACTCGAAGC TCAGGAGCAGTATGGGCTAA 50.0 50.0 58.0 57.8 2188 bp 0.5 58.4