Hotspot

ndhA

SSC · 121796-124098 · 2303 bp

Pi 0.456

MarkerSeek score 78.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2303
variable_sites: 865
pi: 0.456
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TTCGTCCACCGGGTTTTATC	CGGCCCAAAGCAATTTGATT	50.0	45.0	57.9	57.9	2937 bp	0.5	58.4
ndhA_p2	GCTTCGAGCTTCCGGAATAA	CGGACGGTTTGTTGACCATA	50.0	50.0	58.1	58.2	2948 bp	0.5	57.9
ndhA_p3	CGTCCACCGGGTTTTATCAA	CGGCCCAAAGCAATTTGATT	50.0	45.0	58.2	57.9	2935 bp	0.5	57.7
ndhA_p4	TTCGTCCACCGGGTTTTATC	CGGACGGTTTGTTGACCATA	50.0	50.0	57.9	58.2	2556 bp	0.5	57.6
ndhA_p5	TGGGGTTTATCAGGACCGAT	CGGACGGTTTGTTGACCATA	50.0	50.0	58.1	58.2	2968 bp	0.5	57.5