Hotspot

ccsA

SSC · 128572-129546 · 975 bp

Pi 0.47

MarkerSeek score 80.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 377
pi: 0.47
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCGACGAAATGGGTGGAAT	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.0	57.9	1857 bp	0.5	58.8
ccsA_p2	TTTTTCGGTTGAGAGGGTGG	TAGACACGCTGCTCTTAGGA	50.0	50.0	58.0	57.9	1486 bp	0.5	58.8
ccsA_p3	CGAAATGGGTGGAATGGCTA	TAGACACGCTGCTCTTAGGA	50.0	50.0	57.9	57.9	1852 bp	0.5	58.6
ccsA_p4	CTCGACGAAATGGGTGGAAT	AAATTGGTAGACACGCTGCT	50.0	45.0	58.0	57.8	1864 bp	0.5	58.3
ccsA_p5	TTTTTCGGTTGAGAGGGTGG	AAATTGGTAGACACGCTGCT	50.0	45.0	58.0	57.8	1493 bp	0.5	58.3