Hotspot

trnL-UAG-rpl32

SSC · 129739-130432 · 694 bp

Back to Baphia

Pi 0.433
MarkerSeek score 80.90
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
694
variable_sites
257
pi
0.433
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 ATGGGATTGTAGAGCGGGTA ACTAGTTGGATGGCAGTTCC 50.0 50.0 57.9 57.2 1474 bp 0.5 52.9
trnL-UAG-rpl32_p2 TCCTAAGAGCAGCGTGTCTA ACTAGTTGGATGGCAGTTCC 50.0 50.0 57.9 57.2 919 bp 0.5 52.7
trnL-UAG-rpl32_p3 AGCAGCGTGTCTACCAATTT ACTAGTTGGATGGCAGTTCC 45.0 50.0 57.8 57.2 912 bp 0.5 52.2
trnL-UAG-rpl32_p4 AAGACCCGAAGTAGCAAAGC ACTAGTTGGATGGCAGTTCC 50.0 50.0 58.2 57.2 1423 bp 0.5 52.2
trnL-UAG-rpl32_p5 CCGAAGTAGCAAAGCCTTGA ACTAGTTGGATGGCAGTTCC 50.0 50.0 58.2 57.2 1418 bp 0.5 52.2