Hotspot

psbK-trnQ-UUG

LSC · 56779-57514 · 736 bp

Back to Baphia

Pi 0.122
MarkerSeek score 84.70
Primer available yes
Label psbK-trnQ-UUG

Feature Metadata

label_name
psbK-trnQ-UUG
feature_type
igs
region
LSC
length_bp
736
variable_sites
83
pi
0.122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-trnQ-UUG_p1 GGTCCGGGTTTTTCTTGTCT GTCTTTCAAGTCGCACGTTG 50.0 50.0 58.0 58.0 1623 bp 0.5 59.8
psbK-trnQ-UUG_p2 GGTCCGGGTTTTTCTTGTCT CCCTCTTCCTGTCCCCTAAA 50.0 55.0 58.0 58.0 1750 bp 0.5 59.6
psbK-trnQ-UUG_p3 AGCAGCTTGCCAAACAAAAG GTCTTTCAAGTCGCACGTTG 45.0 50.0 58.1 58.0 1454 bp 0.5 59.5
psbK-trnQ-UUG_p4 CTTACAGCAGCTTGCCAAAC GTCTTTCAAGTCGCACGTTG 50.0 50.0 57.9 58.0 1459 bp 0.5 59.5
psbK-trnQ-UUG_p5 GGTCCGGGTTTTTCTTGTCT ACCTCCGAATAACAGGACCA 50.0 50.0 58.0 58.1 1129 bp 0.5 59.4