Hotspot

trnG-GCC-psbZ

LSC · 25196-25984 · 789 bp

Back to Baphia

Pi 0.0896
MarkerSeek score 81.90
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
789
variable_sites
66
pi
0.0896
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGCCGATCCTCAAAAGAAGA TGGGCTCGGCTATATTACCA 50.0 50.0 58.0 58.0 2171 bp 0.5 59.8
trnG-GCC-psbZ_p2 TCGCCGATCCTCAAAAGAAG TGGGCTCGGCTATATTACCA 50.0 50.0 58.0 58.0 2172 bp 0.5 59.8
trnG-GCC-psbZ_p3 CGCCGATCCTCAAAAGAAGA CAACCACTCAGCCATCTCTC 50.0 55.0 58.0 58.0 2009 bp 0.5 59.7
trnG-GCC-psbZ_p4 TCGCCGATCCTCAAAAGAAG CAACCACTCAGCCATCTCTC 50.0 55.0 58.0 58.0 2010 bp 0.5 59.7
trnG-GCC-psbZ_p5 CGTTGTTTTTCGACCGGAAG TGGGCTCGGCTATATTACCA 50.0 50.0 58.0 58.0 2057 bp 0.5 59.7