Hotspot

trnS-GGA-ycf3

LSC · 16808-17074 · 267 bp

Back to Baphia

Pi 0.0951
MarkerSeek score 81.20
Primer available yes
Label trnS-GGA-ycf3

Feature Metadata

label_name
trnS-GGA-ycf3
feature_type
igs
region
LSC
length_bp
267
variable_sites
25
pi
0.0951
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GGA-ycf3_p1 TTCCAATGCTACGCCTTGAA TTCTCCTGAAGTTGTCGGAA 45.0 45.0 57.8 56.4 397-401 bp 1.0 71.6
trnS-GGA-ycf3_p2 TTCCAATGCTACGCCTTGAA TTTCTCCTGAAGTTGTCGGA 45.0 45.0 57.8 56.4 398-402 bp 1.0 71.6
trnS-GGA-ycf3_p3 TTCCAATGCTACGCCTTGAA TCTCCTGAAGTTGTCGGAAT 45.0 45.0 57.8 56.2 396-400 bp 1.0 69.8
trnS-GGA-ycf3_p4 GTGCGGAAAGAGAGGGATTC TTCTCCTGAAGTTGTCGGAA 55.0 45.0 58.3 56.4 450-454 bp 1.0 69.7
trnS-GGA-ycf3_p5 GTGCGGAAAGAGAGGGATTC TTTCTCCTGAAGTTGTCGGA 55.0 45.0 58.3 56.4 451-455 bp 1.0 69.7