Hotspot

tatC

Genome · 115190-115969 · 780 bp

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Pi 0.182
MarkerSeek score 81.10
Primer available yes
Label tatC

Feature Metadata

label_name
tatC
feature_type
gene
region
Genome
length_bp
780
variable_sites
139
pi
0.182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tatC_p1 TTGAGGCAGTCCGATAAAGC GGTAATACTGCCTGAGGTGC 50.0 55.0 58.0 58.0 1721 bp 0.5 59.4
tatC_p2 TTGAGGCAGTCCGATAAAGC GTAATACTGCCTGAGGTGCC 50.0 55.0 58.0 58.0 1720 bp 0.5 59.4
tatC_p3 TTGAGGCAGTCCGATAAAGC GTGCCTCAATTTCTACCGGT 50.0 50.0 58.0 57.9 1705 bp 0.5 58.9
tatC_p4 CTTGCTGCTCTGCTTGAGTA GGTAATACTGCCTGAGGTGC 50.0 55.0 57.9 58.0 1751 bp 0.5 58.9
tatC_p5 CTTGCTGCTCTGCTTGAGTA GTAATACTGCCTGAGGTGCC 50.0 55.0 57.9 58.0 1750 bp 0.5 58.9