Hotspot

sufB

Genome · 85976-87439 · 1464 bp

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Pi 0.156
MarkerSeek score 79.10
Primer available yes
Label sufB

Feature Metadata

label_name
sufB
feature_type
gene
region
Genome
length_bp
1464
variable_sites
228
pi
0.156
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
sufB_p1 TGTATTAAAGGGGATCGGCG AGGCCGGAATCTGTTTCATC 50.0 50.0 57.5 58.0 2651 bp 0.5 55.4
sufB_p2 TGTATTAAAGGGGATCGGCG GGCCGGAATCTGTTTCATCT 50.0 50.0 57.5 58.0 2650 bp 0.5 55.4
sufB_p3 GTATTAAAGGGGATCGGCGT AGGCCGGAATCTGTTTCATC 50.0 50.0 57.5 58.0 2650 bp 0.5 55.3
sufB_p4 GTATTAAAGGGGATCGGCGT GGCCGGAATCTGTTTCATCT 50.0 50.0 57.5 58.0 2649 bp 0.5 55.3
sufB_p5 TGTATTAAAGGGGATCGGCG TCTAGATTTTGCGCAAGGCT 50.0 45.0 57.5 57.9 2834 bp 0.5 54.8