Hotspot

orf382b

Genome · 173446-174270 · 825 bp

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Pi 0.294
MarkerSeek score 79.00
Primer available yes
Label orf382b

Feature Metadata

label_name
orf382b
feature_type
gene
region
Genome
length_bp
825
variable_sites
242
pi
0.294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf382b_p1 TCTCTCTCCGTGTCGAGAAA TTGTCCCACAGAAGGAGCTA 50.0 50.0 57.8 58.0 1273 bp 0.5 58.4
orf382b_p2 CGCCTGGTGTTATTCTGTGT TTGTCCCACAGAAGGAGCTA 50.0 50.0 58.2 58.0 1539 bp 0.5 58.3
orf382b_p3 CTTGCTTTTGGTTTCGCACT TTGTCCCACAGAAGGAGCTA 45.0 50.0 57.8 58.0 1631 bp 0.5 58.1
orf382b_p4 GGGTTCGAATCCCTCTCTCT TTGTCCCACAGAAGGAGCTA 55.0 50.0 58.2 58.0 1286 bp 0.5 58.1
orf382b_p5 AATCCCTCTCTCTCCGTGTC TTGTCCCACAGAAGGAGCTA 55.0 50.0 58.2 58.0 1279 bp 0.5 58.0