Hotspot

orf121

Genome · 67977-68342 · 366 bp

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Pi 0.344
MarkerSeek score 73.90
Primer available yes
Label orf121

Feature Metadata

label_name
orf121
feature_type
gene
region
Genome
length_bp
366
variable_sites
126
pi
0.344
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
orf121_p1 TATCCCTAAGACACGCAGCA ATTCCTGAACAGCCACCTTG 50.0 50.0 58.5 58.1 1605 bp 0.5 55.2
orf121_p2 TATCCCTAAGACACGCAGCA CATTCCTGAACAGCCACCTT 50.0 50.0 58.5 58.1 1606 bp 0.5 55.2
orf121_p3 TCCCTAAGACACGCAGCATA ATTCCTGAACAGCCACCTTG 50.0 50.0 58.5 58.1 1603 bp 0.5 55.2
orf121_p4 TCCCTAAGACACGCAGCATA CATTCCTGAACAGCCACCTT 50.0 50.0 58.5 58.1 1604 bp 0.5 55.2
orf121_p5 CCCTAAGACACGCAGCATAT ATTCCTGAACAGCCACCTTG 50.0 50.0 57.5 58.1 1602 bp 0.5 55.1