Hotspot

dnaB

Genome · 170483-172192 · 1710 bp

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Pi 0.279
MarkerSeek score 78.70
Primer available yes
Label dnaB

Feature Metadata

label_name
dnaB
feature_type
gene
region
Genome
length_bp
1710
variable_sites
475
pi
0.279
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
dnaB_p1 GATCCTCGTGTCACCAGTTC AAAACTGGAGACGGGGAAAC 55.0 50.0 58.0 58.0 2053 bp 0.5 59.8
dnaB_p2 TCAGTTGGTAGAGCAGTGGA AAAACTGGAGACGGGGAAAC 50.0 50.0 58.0 58.0 2078 bp 0.5 59.7
dnaB_p3 GATCCTCGTGTCACCAGTTC TGTTACAGTTGCAGCAGGTT 55.0 45.0 58.0 57.9 2246 bp 0.5 59.6
dnaB_p4 GATCCTCGTGTCACCAGTTC TTGTTACAGTTGCAGCAGGT 55.0 45.0 58.0 57.9 2247 bp 0.5 59.6
dnaB_p5 TCAGTTGGTAGAGCAGTGGA TGTTACAGTTGCAGCAGGTT 50.0 45.0 58.0 57.9 2271 bp 0.5 59.5