Hotspot

atpB

Genome · 108936-110363 · 1428 bp

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Pi 0.137
MarkerSeek score 79.20
Primer available yes
Label atpB

Feature Metadata

label_name
atpB
feature_type
gene
region
Genome
length_bp
1428
variable_sites
195
pi
0.137
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB_p1 CCACGTCAACTATGCCAAGA AATTGAGTGGCTGCTTCCAA 50.0 45.0 57.9 58.0 2431 bp 0.5 59.3
atpB_p2 CCACGTCAACTATGCCAAGA ATTGAGTGGCTGCTTCCAAA 50.0 45.0 57.9 58.0 2430 bp 0.5 59.3
atpB_p3 CCACGTCAACTATGCCAAGA TGAGTGGCTGCTTCCAAATT 50.0 45.0 57.9 58.0 2428 bp 0.5 59.3
atpB_p4 CCACGTCAACTATGCCAAGA TTGAGTGGCTGCTTCCAAAT 50.0 45.0 57.9 58.0 2429 bp 0.5 59.3
atpB_p5 TCCACGTCAACTATGCCAAG TGAGTGGCTGCTTCCAAATT 50.0 45.0 57.9 58.0 2429 bp 0.5 59.3