Hotspot

trnK-UUU

LSC · 3917-3954 · 38 bp

Back to Bambusa

Pi 0.0083

MarkerSeek score 56.70

Primer available yes

Label trnK-UUU

Feature Metadata

label_name: trnK-UUU
feature_type: tRNA
region: LSC
length_bp: 38
variable_sites: 4
pi: 0.0083
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU_p1	TGAGGATCAGTCGTGGTCTT	CGTGCGGTGTAATTCCATTG	50.0	50.0	58.1	57.8	180-185 bp	1.0	82.3
trnK-UUU_p2	AGGATCAGTCGTGGTCTTCT	CGTGCGGTGTAATTCCATTG	50.0	50.0	57.8	57.8	178-183 bp	1.0	81.2
trnK-UUU_p3	GAGGATCAGTCGTGGTCTTC	CGTGCGGTGTAATTCCATTG	55.0	50.0	57.4	57.8	179-184 bp	1.0	78.4
trnK-UUU_p4	TGAGGATCAGTCGTGGTCTT	AATGTTTTGACAGGAGCGTG	50.0	45.0	58.1	56.9	196-201 bp	1.0	74.9
trnK-UUU_p5	TGAGGATCAGTCGTGGTCTT	CAATGTTTTGACAGGAGCGT	50.0	45.0	58.1	56.9	197-202 bp	1.0	74.8