Hotspot

trnF-GAA-ndhJ

LSC · 49697-50277 · 581 bp

Back to Bambusa

Pi 0.00346

MarkerSeek score 47.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 581
variable_sites: 15
pi: 0.00346
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	690-727 bp	1.0	80.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	680-717 bp	1.0	80.1
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TCCACGCCTTAAACGTATCC	50.0	50.0	58.1	57.7	717-754 bp	1.0	79.2
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CCACGCCTTAAACGTATCCT	50.0	50.0	58.1	57.7	716-753 bp	1.0	79.1
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	721-758 bp	1.0	78.4