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trnD-GUC-psbM

LSC · 17106-18161 · 1056 bp

Back to Bambusa

Pi 0.00356

MarkerSeek score 44.60

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1056
variable_sites: 33
pi: 0.00356
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TAGGTATGCCATACACCCCG	AGAATGAACAGTGCAGTAGC	55.0	45.0	58.7	55.8	1199-1206 bp	1.0	59.3
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	50.0	45.0	58.5	55.8	1167-1168 bp	0.972973	58.0
trnD-GUC-psbM_p3	TAGGTATGCCATACACCCCG	AGGAACTAGAATGAACAGTGCA	55.0	40.9	58.7	57.4	1206-1213 bp	1.0	56.1
trnD-GUC-psbM_p4	TTCAATTGGTCAGAGCACCG	AGGAACTAGAATGAACAGTGCA	50.0	40.9	58.5	57.4	1174-1175 bp	0.972973	54.8
trnD-GUC-psbM_p5	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	50.0	45.0	57.0	55.8	1168-1169 bp	0.972973	53.8