Hotspot

trnG-UCC-trnT-GGU

LSC · 13895-15855 · 1961 bp

Back to Bambusa

Pi 0.00757
MarkerSeek score 56.60
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1961
variable_sites
74
pi
0.00757
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 38.1 52.4 55.5 58.1 2022-2228 bp 1.0 48.7
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT GACTTACGTCTTACCATGGCA 38.1 47.6 55.5 57.8 2035-2241 bp 1.0 48.0
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT TGACTTACGTCTTACCATGGC 38.1 47.6 55.5 57.8 2036-2242 bp 1.0 48.0
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2025-2231 bp 1.0 47.7
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2024-2230 bp 1.0 47.7