Hotspot

rps11

LSC · 78048-78464 · 417 bp

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Pi 0

MarkerSeek score 33.70

Primer available yes

Label rps11

Feature Metadata

label_name: rps11
feature_type: gene
region: LSC
length_bp: 417
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11_p1	AGCGTCCGAGTAGATACTGT	ACTCACCAAAGGACTAAACGT	50.0	42.9	57.7	57.2	638 bp	1.0	62.7
rps11_p2	AGCGTCCGAGTAGATACTGT	AGACTCACCAAAGGACTAAACG	50.0	45.5	57.7	58.0	640 bp	1.0	61.3
rps11_p3	CAGCGTCCGAGTAGATACTG	ACTCACCAAAGGACTAAACGT	55.0	42.9	57.3	57.2	639 bp	1.0	60.0
rps11_p4	AGCGTCCGAGTAGATACTGT	ATCAGACTCACCAAAGGACT	50.0	45.0	57.7	55.8	643 bp	1.0	59.5
rps11_p5	AGCGTCCGAGTAGATACTGT	GACTCACCAAAGGACTAAACGT	50.0	45.5	57.7	58.3	639 bp	1.0	59.2