Hotspot

rpl20-rps12

LSC · 68265-69005 · 741 bp

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Pi 0.00135

MarkerSeek score 59.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 1
pi: 0.00135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGAATGAGACCCCCGAAAAC	CGCCGAGGAACATGTACTAG	50.0	55.0	57.8	57.9	855 bp	1.0	77.6
rpl20-rps12_p2	AGACCCCCGAAAACTTGATG	CGCCGAGGAACATGTACTAG	50.0	55.0	57.8	57.9	849 bp	1.0	77.5
rpl20-rps12_p3	ATGAGACCCCCGAAAACTTG	CGCCGAGGAACATGTACTAG	50.0	55.0	57.8	57.9	852 bp	1.0	77.5
rpl20-rps12_p4	TGAATGAGACCCCCGAAAAC	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	907 bp	1.0	76.9
rpl20-rps12_p5	AGACCCCCGAAAACTTGATG	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	901 bp	1.0	76.9