Hotspot

rbcL-accD

LSC · 56180-56869 · 690 bp

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Pi 0.0029

MarkerSeek score 60.40

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 2
pi: 0.0029
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGCAAGTGGAGTCCTGAAC	TTTAGCCCATACCTGCGTTC	50.0	50.0	58.0	58.0	858 bp	1.0	79.7
rbcL-accD_p2	TTGCAAGTGGAGTCCTGAAC	CTTTAGCCCATACCTGCGTT	50.0	50.0	58.0	58.0	859 bp	1.0	79.6
rbcL-accD_p3	TTGCAAGTGGAGTCCTGAAC	TAGCCCATACCTGCGTTCTA	50.0	50.0	58.0	57.9	856 bp	1.0	79.3
rbcL-accD_p4	CTTGCAAGTGGAGTCCTGAA	TTTAGCCCATACCTGCGTTC	50.0	50.0	57.7	58.0	859 bp	1.0	77.8
rbcL-accD_p5	CTTGCAAGTGGAGTCCTGAA	CTTTAGCCCATACCTGCGTT	50.0	50.0	57.7	58.0	860 bp	1.0	77.8