Hotspot

trnT-UGU-trnL-UAA

LSC · 45945-46642 · 698 bp

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Pi 0.00143
MarkerSeek score 59.60
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
698
variable_sites
1
pi
0.00143
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG AGTTGGTTTCCCGCACTAAG 55.0 50.0 57.9 58.1 802 bp 1.0 78.2
trnT-UGU-trnL-UAA_p2 GCGATGCTCTAACCTCTGAG CTTAGTTGGTTTCCCGCACT 55.0 50.0 57.9 58.1 805 bp 1.0 78.2
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG TCACTTAGTTGGTTTCCCGC 55.0 50.0 57.9 58.1 808 bp 1.0 78.1
trnT-UGU-trnL-UAA_p4 TAGGAAAAAGCCGGCTATCG AGTTGGTTTCCCGCACTAAG 50.0 50.0 57.8 58.1 853 bp 1.0 77.4
trnT-UGU-trnL-UAA_p5 TAGGAAAAAGCCGGCTATCG CTTAGTTGGTTTCCCGCACT 50.0 50.0 57.8 58.1 856 bp 1.0 77.4