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psbM-trnD-GUC

LSC · 29820-30366 · 547 bp

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Pi 0.00183

MarkerSeek score 58.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 1
pi: 0.00183
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TAAGCAGAAAGGCGGTAGGA	CCCCTATTTCTTTTCCCCGG	50.0	55.0	58.4	57.9	708-709 bp	1.0	76.2
psbM-trnD-GUC_p2	TAAGCAGAAAGGCGGTAGGA	CCCTATTTCTTTTCCCCGGG	50.0	55.0	58.4	57.9	707-708 bp	1.0	76.2
psbM-trnD-GUC_p3	ATAAGCAGAAAGGCGGTAGG	CCCCTATTTCTTTTCCCCGG	50.0	55.0	57.4	57.9	709-710 bp	1.0	74.7
psbM-trnD-GUC_p4	ATAAGCAGAAAGGCGGTAGG	CCCTATTTCTTTTCCCCGGG	50.0	55.0	57.4	57.9	708-709 bp	1.0	74.7
psbM-trnD-GUC_p5	TAAGCAGAAAGGCGGTAGGA	TCCCCGGGATTGTAGTTCAA	50.0	50.0	58.4	58.3	695-696 bp	1.0	74.0