Hotspot

rpoB-trnC-GCA

LSC · 26863-27959 · 1097 bp

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Pi 0.000912

MarkerSeek score 59.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1097
variable_sites: 1
pi: 0.000912
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CATTGCCTCATTTGCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1186 bp	1.0	77.5
rpoB-trnC-GCA_p2	GTTCTTCTGTCAAACCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1262 bp	1.0	77.4
rpoB-trnC-GCA_p3	AGTTCTTCTGTCAAACCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.7	58.1	1263 bp	1.0	77.4
rpoB-trnC-GCA_p4	CATTGCCTCATTTGCATCCC	TTTGGGTAATCAGGCGACAC	50.0	50.0	57.8	58.2	1206 bp	1.0	76.7
rpoB-trnC-GCA_p5	GTTCTTCTGTCAAACCCCGA	TTTGGGTAATCAGGCGACAC	50.0	50.0	57.8	58.2	1282 bp	1.0	76.6