Hotspot

psaC

SSC · 122951-123196 · 246 bp

Back to Balanops

Pi 0.0122

MarkerSeek score 44.90

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 3
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GATGCCCAAAGCTCCCATTA	TCTCATGAGTCGATCCGGAA	50.0	50.0	57.9	57.9	1064-1069 bp	1.0	79.8
psaC_p2	GAGTACGGGTTTTTGTCGGT	TCTCATGAGTCGATCCGGAA	50.0	50.0	58.1	57.9	443-448 bp	1.0	79.4
psaC_p3	AAAATTAAGGAACCCGCGGA	TCTCATGAGTCGATCCGGAA	45.0	50.0	57.8	57.9	574-579 bp	1.0	79.3
psaC_p4	TGGAATAATCGGGAATCGCG	TCTCATGAGTCGATCCGGAA	50.0	50.0	58.2	57.9	853-858 bp	1.0	78.8
psaC_p5	GATGCCCAAAGCTCCCATTA	ACCGGCTATTGTTTCGTCAA	50.0	45.0	57.9	57.8	1255-1260 bp	1.0	78.7