Hotspot

rps15-ycf1

SSC · 129337-129741 · 405 bp

Back to Balanops

Pi 0.0205

MarkerSeek score 57.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 405
variable_sites: 8
pi: 0.0205
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCTTGTGAAATAACTGAGATGAATGA	ACCAACAATGCCAGTCGATT	30.8	45.0	56.9	58.1	495-509 bp	1.0	43.5
rps15-ycf1_p2	TCTTGTGAAATAACTGAGATGA	ACCAACAATGCCAGTCGATT	31.8	45.0	52.9	58.1	495-509 bp	1.0	43.5
rps15-ycf1_p3	TGTGAAATAACTGAGATGAATGA	ACCAACAATGCCAGTCGATT	30.4	45.0	53.8	58.1	492-506 bp	1.0	43.5
rps15-ycf1_p4	TCTTGTGAAATAACTGAGATGAA	ACCAACAATGCCAGTCGATT	30.4	45.0	53.7	58.1	495-509 bp	1.0	43.5
rps15-ycf1_p5	TTCTTGTGAAATAACTGAGATGA	ACCAACAATGCCAGTCGATT	30.4	45.0	53.7	58.1	496-510 bp	1.0	43.5