Hotspot

ndhI-ndhA

SSC · 125479-125528 · 50 bp

Back to Balanops

Pi 0.02

MarkerSeek score 64.00

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 50
variable_sites: 1
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	ACTTTGGCCAATGTACCTCG	AGGTGGACTTTACCTCGACT	50.0	50.0	58.2	57.7	275 bp	1.0	76.4
ndhI-ndhA_p2	GCAGCTCGTATGGTTTGTTG	AGGTGGACTTTACCTCGACT	50.0	50.0	57.8	57.7	256 bp	1.0	75.9
ndhI-ndhA_p3	ATGTACCTCGCAGCTCGTAT	AGGTGGACTTTACCTCGACT	50.0	50.0	58.7	57.7	265 bp	1.0	72.4
ndhI-ndhA_p4	GCTCGTATGGTTTGTTGACC	AGGTGGACTTTACCTCGACT	50.0	50.0	57.1	57.7	253 bp	1.0	70.7
ndhI-ndhA_p5	AATGTACCTCGCAGCTCGTA	AGGTGGACTTTACCTCGACT	50.0	50.0	58.9	57.7	266 bp	1.0	70.7