Hotspot

rpl32-trnL-UAG

SSC · 118835-119951 · 1117 bp

Back to Balanops

Pi 0.0136
MarkerSeek score 67.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1117
variable_sites
15
pi
0.0136
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGAAGGGATATTGGACAGCG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 1256-1341 bp 1.0 80.9
rpl32-trnL-UAG_p2 GGAAGGGATATTGGACAGCG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 1257-1342 bp 1.0 80.9
rpl32-trnL-UAG_p3 GGAAGGGATATTGGACAGCG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.1 57.9 1266-1351 bp 1.0 80.0
rpl32-trnL-UAG_p4 GGGAAGGGATATTGGACAGC AGCGTGTCTACCGATTTCAC 55.0 50.0 57.7 58.0 1257-1342 bp 1.0 79.6
rpl32-trnL-UAG_p5 GGGAAGGGATATTGGACAGC CAGCGTGTCTACCGATTTCA 55.0 50.0 57.7 58.0 1258-1343 bp 1.0 79.6