Hotspot

rps19-rpl2

IRb · 88934-88993 · 60 bp

Back to Balanops

Pi 0.0167

MarkerSeek score 62.10

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 60
variable_sites: 1
pi: 0.0167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TCCGCCTTTGTGTTAAGCTT	GGGCCCCAATTGGTAGAAAA	45.0	50.0	58.0	58.1	259-268 bp	1.0	79.7
rps19-rpl2_p2	TTCCGCCTTTGTGTTAAGCT	GGGCCCCAATTGGTAGAAAA	45.0	50.0	58.0	58.1	260-269 bp	1.0	79.7
rps19-rpl2_p3	TCCGCCTTTGTGTTAAGCTT	TTGATTCTTCGTCGCCGTAG	45.0	50.0	58.0	58.1	167-176 bp	1.0	79.5
rps19-rpl2_p4	TTCCGCCTTTGTGTTAAGCT	TTGATTCTTCGTCGCCGTAG	45.0	50.0	58.0	58.1	168-177 bp	1.0	79.5
rps19-rpl2_p5	TGGTAGATGCTCGAGACCAT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	57.9	58.1	297-306 bp	1.0	79.2