Hotspot

rpl20-rps12

LSC · 73469-74270 · 802 bp

Back to Balanops

Pi 0.00873
MarkerSeek score 63.80
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
802
variable_sites
7
pi
0.00873
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGCGCTATATATCCTCGT CGCCGAGGAACATGTACTAG 50.0 55.0 58.0 57.9 863-865 bp 1.0 79.4
rpl20-rps12_p2 TCCGCGCTATATATCCTCGT AAATGTCACCAAATCCCCCG 50.0 50.0 58.0 58.4 903-905 bp 1.0 76.8
rpl20-rps12_p3 CCGCGCTATATATCCTCGTC CGCCGAGGAACATGTACTAG 55.0 55.0 57.6 57.9 862-864 bp 1.0 76.6
rpl20-rps12_p4 TCCGCGCTATATATCCTCGT GCCGAGGAACATGTACTAGG 50.0 55.0 58.0 57.5 862-864 bp 1.0 76.3
rpl20-rps12_p5 CTCCGCGCTATATATCCTCG CGCCGAGGAACATGTACTAG 55.0 55.0 57.3 57.9 864-866 bp 1.0 74.3