Hotspot

petA-psbJ

LSC · 67034-68071 · 1038 bp

Back to Balanops

Pi 0.00974

MarkerSeek score 65.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1038
variable_sites: 10
pi: 0.00974
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CATTACGTGTCCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1180-1194 bp	1.0	77.1
petA-psbJ_p2	GACCCATTACGTGTCCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1184-1198 bp	1.0	76.4
petA-psbJ_p3	TTACGTGTCCAAGGCCTTTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.6	57.6	1178-1192 bp	1.0	75.7
petA-psbJ_p4	TACGTGTCCAAGGCCTTTTG	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.4	57.6	1177-1191 bp	1.0	75.3
petA-psbJ_p5	TGTCCAAGGCCTTTTGTTCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.5	57.6	1173-1187 bp	1.0	74.9