Hotspot

psbZ-trnG-GCC

LSC · 39158-39623 · 466 bp

Back to Balanops

Pi 0.0172

MarkerSeek score 65.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 466
variable_sites: 8
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.0	57.8	620-629 bp	1.0	79.5
psbZ-trnG-GCC_p2	TCCTGATGGTTGGTCGAGTA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	616-625 bp	1.0	77.9
psbZ-trnG-GCC_p3	TTCTCCTGATGGTTGGTCGA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	619-628 bp	1.0	76.8
psbZ-trnG-GCC_p4	TCTCCTGATGGTTGGTCGAG	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.8	57.8	618-627 bp	1.0	73.2
psbZ-trnG-GCC_p5	CTTCTCCTGATGGTTGGTCG	CGGATAGCGGGAATCGAAC	55.0	57.9	58.0	58.2	642-651 bp	1.0	71.3