Hotspot

psbC-trnS-UGA

LSC · 38312-38532 · 221 bp

Back to Balanops

Pi 0.0317

MarkerSeek score 64.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 221
variable_sites: 7
pi: 0.0317
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	58.4	58.1	368-375 bp	1.0	78.0
psbC-trnS-UGA_p2	TAGGTCATTTATGGCACGCG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.4	58.1	402-409 bp	1.0	77.5
psbC-trnS-UGA_p3	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	367-374 bp	1.0	77.5
psbC-trnS-UGA_p4	AGCTGCAGCAGGATTTGAAA	GATGGCCGAGTGGTTGATAG	45.0	55.0	58.4	58.1	379-386 bp	1.0	77.4
psbC-trnS-UGA_p5	AGCTGCAGCAGGATTTGAAA	TTTAGGAGAGATGGCCGAGT	45.0	50.0	58.4	57.8	388-395 bp	1.0	77.1