Hotspot

psbM-trnD-GUC

LSC · 31613-32843 · 1231 bp

Back to Balanops

Pi 0.00656

MarkerSeek score 63.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1231
variable_sites: 8
pi: 0.00656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGCAAAAAGGCAGTAGGAACA	TTCAATTGGTCAGAGCACCG	42.9	50.0	58.1	58.5	1363 bp	1.0	68.8
psbM-trnD-GUC_p2	GCAAAAAGGCAGTAGGAACA	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.5	58.5	1362 bp	1.0	65.6
psbM-trnD-GUC_p3	AGCAAAAAGGCAGTAGGAACA	GTTCAATTGGTCAGAGCACC	42.9	50.0	58.1	57.0	1364 bp	1.0	64.7
psbM-trnD-GUC_p4	GCAAAAAGGCAGTAGGAACAA	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.3	58.5	1362 bp	1.0	63.5
psbM-trnD-GUC_p5	AGCAAAAAGGCAGTAGGAAC	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.2	58.5	1363 bp	1.0	63.2