Hotspot

petN-psbM

LSC · 30538-31507 · 970 bp

Back to Balanops

Pi 0.00211

MarkerSeek score 62.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 970
variable_sites: 2
pi: 0.00211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGTTCCTACTGCCTTTTTGCT	50.0	42.9	58.2	58.1	1093-1111 bp	1.0	71.9
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1114-1132 bp	1.0	69.1
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TGTTCCTACTGCCTTTTTGC	50.0	45.0	58.2	56.5	1093-1111 bp	1.0	68.7
petN-psbM_p4	TATGGGGAAGAAGTGGCCTC	TGTTCCTACTGCCTTTTTGCT	55.0	42.9	58.8	58.1	1033-1051 bp	1.0	66.9
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	TTGTTCCTACTGCCTTTTTGC	50.0	42.9	58.2	57.3	1094-1112 bp	1.0	66.6