Hotspot

atpF-atpH

LSC · 14150-14768 · 619 bp

Back to Balanops

Pi 0.00826

MarkerSeek score 65.70

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 619
variable_sites: 5
pi: 0.00826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	GGACTGGTTGTTGCATTAGC	50.0	50.0	58.0	57.4	703-708 bp	1.0	76.8
atpF-atpH_p2	CAGTGACCCAAGGAAACGAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	768-773 bp	1.0	76.2
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	GAGGCGGAGGGAAAAATACG	50.0	55.0	58.0	58.7	772-777 bp	1.0	76.0
atpF-atpH_p4	CAGTGACCCAAGGAAACGAA	GCGGAGGGAAAAATACGAGG	50.0	55.0	58.0	58.7	769-774 bp	1.0	76.0
atpF-atpH_p5	CAGTGACCCAAGGAAACGAA	GGCGGAGGGAAAAATACGAG	50.0	55.0	58.0	58.7	770-775 bp	1.0	76.0