Hotspot

trnS-GCU-trnG-UCC

LSC · 8803-9735 · 933 bp

Back to Balanops

Pi 0.0156

MarkerSeek score 64.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 933
variable_sites: 14
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	GAATATTAGCCGAGGGATCCC	50.0	52.4	58.0	57.5	1012-1044 bp	1.0	71.3
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	GGAATATTAGCCGAGGGATCC	50.0	52.4	58.0	57.5	1013-1045 bp	1.0	71.3
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	GAATATTAGCCGAGGGATCCC	50.0	52.4	58.0	57.5	1011-1043 bp	1.0	71.3
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	GGAATATTAGCCGAGGGATCC	50.0	52.4	58.0	57.5	1012-1044 bp	1.0	71.2
trnS-GCU-trnG-UCC_p5	ATCAACGGAACCGGAAAGAG	GAATATTAGCCGAGGGATCCC	50.0	52.4	57.9	57.5	1076-1108 bp	1.0	70.3