Hotspot

psbI-trnS-GCU

LSC · 8578-8714 · 137 bp

Back to Balanops

Pi 0.0292

MarkerSeek score 69.10

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 4
pi: 0.0292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.3	58.0	245-248 bp	1.0	79.1
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.3	58.0	246-249 bp	1.0	79.1
psbI-trnS-GCU_p3	ATGATCCAGGGCGTAATCCT	TTTCAATTGGGAGAGGTGGC	50.0	50.0	58.3	58.1	269-272 bp	1.0	78.4
psbI-trnS-GCU_p4	ATGATCCAGGGCGTAATCCT	GGTGGCTGAGTGGACTAAAG	50.0	55.0	58.3	57.9	255-258 bp	1.0	78.3
psbI-trnS-GCU_p5	ATGATCCAGGGCGTAATCCT	AGGTGGCTGAGTGGACTAAA	50.0	50.0	58.3	58.3	256-259 bp	1.0	77.0