Hotspot

trnH-GUG-psbA

LSC · 232-677 · 446 bp

Back to Balanops

Pi 0.00897

MarkerSeek score 55.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 4
pi: 0.00897
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	512-534 bp	1.0	79.1
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	526-548 bp	1.0	79.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	525-547 bp	1.0	79.0
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	527-549 bp	1.0	79.0
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	TAGACCTAGCTGCTGTCGAA	55.0	50.0	58.1	57.9	508-530 bp	1.0	78.6