Hotspot

ndhF

SSC · 114092-116305 · 2214 bp

Back to Bakerantha

Pi NA

MarkerSeek score 19.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 19.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACTTTTCTTTGCTCTAAAATTCT	GGTGAATCGAGAACAATTTGCA	26.1	40.9	52.8	57.8	2288 bp	0.333333	13.3
ndhF_p2	ACTTTTCTTTGCTCTAAAATTCT	TGGTGAATCGAGAACAATTTGC	26.1	40.9	52.8	57.8	2289 bp	0.333333	13.3
ndhF_p3	TCTTACTTTTCTTTGCTCTAAAA	GGTGAATCGAGAACAATTTGCA	26.1	40.9	52.3	57.8	2292 bp	0.333333	13.3
ndhF_p4	TCTTACTTTTCTTTGCTCTAAAA	TGGTGAATCGAGAACAATTTGC	26.1	40.9	52.3	57.8	2293 bp	0.333333	13.3
ndhF_p5	TTCTTACTTTTCTTTGCTCTAAA	GGTGAATCGAGAACAATTTGCA	26.1	40.9	52.3	57.8	2293 bp	0.333333	13.3